[inserm-01077352] Nanoscale surface topography reshapes neuronal growth in culture

Neurons are sensitive to topographical cues provided either by in vivo or in vitro environments on the micrometric scale We have explored the role of randomly distributed silicon nanopillars on primary hippocampal neurite elongation and axonal differentiation We observed that neurons adhere on the upper part of nanopillars with a typical distance between adhesion points of about 500 nm These neurons produce fewer neurites elongate faster and differentiate an axon earlier than those grown on flat silicon surfaces Moreover when confronted with a differential surface topography neurons specify an axon preferentially on nanopillars As a whole these results highlight the influence of the physical environment in many aspects of neuronal growth



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