[hal-01078423] Laser line scanning for fluorescence reflectance imaging a phantom study and in vivo validation of the enhancement of contrast and resolution Laser line scanning for fluorescence reflectance imaging a phantom study and in vivo validation of the enhancement of contrast and resolution

Intraoperative fluorescence imaging in reflectance geometry is an attractive imaging modality to non-invasively monitor fluorescence-targeted tumors In some situations this kind of imaging suffers from poor res-olution due to the diffusive nature of photons in tissue The objective of the proposed technique is to tackle this limitation It relies on the scanning of the medium with a laser line illumination and the acquisition of images at each position of excitation The detection scheme proposed takes advantage of the stack of images acquired to enhance the resolution and the contrast of the final image The experimental protocol is described to fully under-stand why we overpass the classical limits and validate the scheme on tissue-like phantoms and in vivo with a preliminary testing The results are compared with those obtained with a classical wide-field illumination pub-lished online Oct 1 2014



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